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中文名称

Goat anti-Mouse IgG1(subclass specific), HRP conjugated

英文名字
Goat anti-Mouse IgG1(subclass specific),HRP conjugated
供应商
Agrisera
产品货号
AS16 3715
产品报价
¥7905.6/1ml
产品说明书
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背景资料
The reactivity of the antiserum is directed to the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG1 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1.000 and 1:10,000 depending on the method used.
应用类型
Dot blot (Dot), ELISA, IF, Immunohistochemistry (paraffin) (IHC), WB
免疫原
Pools of purified homogenous IgG1 isolated from pooled mouse serum. Freund
来源宿主
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反应性
Mouse
保存建议
其他
Agrisera
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